Comparison of Hematocrit Values Using the Microhematocrit Method and the Automatic Hematology Analyzer

Authors

  • Suparyati Akademi Analis Kesehatan Pekalongan
  • Delia Maharani Putri Akademi Analis Kesehatan Pekalongan

DOI:

https://doi.org/10.56359/igj.v4i3.655

Keywords:

Hematocrit, Microhematocrit, Hematology Analyzer, Manual Method, Automatic Method

Abstract

Background & Objective: Hematocrit is the percentage of erythrocyte volume in blood that is important for assessing health status, especially in relation to oxygen transport capacity. Hematocrit tests can be performed manually (microhematocrit) or automatically (hematology analyzer). Differences in the working principles of the two methods can cause variations in results. This study aims to determine the difference in hematocrit values using the microhematocrit method and the automatic hematology analyzer.

Method: This study is analytical in nature with a quantitative approach, using 30 venous blood samples from outpatients at Kraton Pekalongan Regional General Hospital. The examination was performed using the microhematocrit method and the automatic hematology analyzer. The data were analyzed using normality tests and Paired Sample T-Tests using SPSS.

Result: The average hematocrit value using the manual microhematocrit method was 33.33%, while the average value using the automatic hematology analyzer was 32.41%. The statistical test results showed a significant value of 0.075 (P> 0.05), indicating that there was no significant difference between the two methods.

Conclusion: There was no significant difference between the hematocrit values obtained by the microhematocrit method and the automatic hematology analyzer. Both methods can be used interchangeably depending on the availability of equipment and clinical needs.

Downloads

Download data is not yet available.

References

Arini, Fitria Yulfirda, Anik Handayati, Sri Sulami Endah Astuti, and Anita Dwi Anggraini. 2023. “Uji Komparasi Hasil Pemeriksaan Hemoglobin Menggunakan Hematology Analyzer Dan Hemoglobin Meter Pada Pasien Kadar Normal Dan Abnormal Rendah.” Jurnal Penelitian Kesehatan Suara Forikes 14(1): 235–38.

Chairani, Chairani, Vetra Susanto, Siti Monitari, and Marisa Marisa. 2022. “Nilai Hematokrit Pada Pasien Hemodialisa Dengan Metode Mikrohematokrit Dan Automatik.” JURNAL KESEHATAN PERINTIS (Perintis’s Health Journal) 9(2): 89–93. doi:10.33653/jkp.v9i2.872.

Hasanah, Annisa Nur, and Taufik Hidayat. 2024. “Perbedaan Nilai Hematokrit Menggunakan Metode Mikrohematokrit Dengan Metode Automatik Hematology Analyzer Bc-2300.” Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-ilmu Keperawatan, Analis Kesehatan dan Farmasi 24(1). doi:10.36465/jkbth.v24i1.1241.

Hastuti, Fitriana D W I. 2018. “Perbedaan Nilai Hematokrit Metode Mikro Dan Hematology Analyzer Pada Peminum Alkohol.”

Hidayah, Nurul. 2018. “Perbedaan Nilai Hematokrit Darah Kapiler Menggunakan Hematologi Analizer Dengan Manual Mikrohematokrit.” Skripsi: 5–16.

Kesuma, Suryanata, Mahdiah Syumarliyanty, and Agus Rudi Hartono. 2021. “Evaluasi Analitik Hematology Analyzer Diatron Abacus 3 Pada Parameter Hematologi Rutin Di Laboratorium Hematologi Poltekkes Kemenkes Kalimantan Timur.” the Journal of Muhammadiyah Medical Laboratory Technologist 4(1): 1. doi:10.30651/jmlt.v4i1.6467.

Maharani, Rizki. 2017. “PERBEDAAN KADAR HEMATOKRIT METODE MIKRO MENGGUNAKAN DARAH VENA DENGAN VOLUME TABUNG 75% DAN 50%.” https://api.semanticscholar.org/CorpusID:126819102.

Nirwani, Tri Hartati, and Zulfikar Husni Faruq. 2018. “Analisis Akurasi Dan Presisi Alat Hematologi Analyzer ABX PENTRA XL 80 Di Laboratorium Rumah Sakit Roemani Muhammadiyah Semarang.” Manuscript Universitas Muhammadiyah Semarang: 1–9. http://repository.unimus.ac.id.

Nugrahani, Wahyu, Tulus Ariyadi, and Fitri Nuroini. 2018. “Perbedaan Nilai Hematokrit Darah EDTA Metode Autoanalyzer Dan Mikrokapiler Pada Tersangka Demam Berdarah Dengue.” Universitas Muhammadiyah Semarang 1: 1–4. http://repository.unimus.ac.id/3018/1/MANUSCRIPT.pdf.

Nuraeni, Maria. 2020. “Perbandingan Nilai Hematokrit Darah Vena Metode Automatik Dan Darah Kapiler Metode Mikro Hematokrit.” kesehatan saelmakers PERDANA 3(2): 1–6.

Subur Wibowo, and Isnaini Isnaini. 2024. “Pengaruh Variasi Waktu Dan Kecepatan Centrifuge Terhadap Nilai Hematokrit Metode Makrohematokrit.” Jurnal Medika Husada 4(1): 25–35. doi:10.59744/jumeha.v4i1.63.

Yumaroh, Siti. 2020. “Perbedaan Kadar Hematokrit Darah Edta Disimpan Suhu Kamar Dan Suhu Lemari Pendingin Selama 6 Jam Dan 18 Jam.” Jurnal Teknologi Kesehatan 9(3): 1–23.

Downloads

Published

2025-10-01

How to Cite

Suparyati, & Maharani Putri, D. (2025). Comparison of Hematocrit Values Using the Microhematocrit Method and the Automatic Hematology Analyzer. INDOGENIUS, 4(3), 760–767. https://doi.org/10.56359/igj.v4i3.655

Issue

Section

Articles