Literature Review : The Application of High-Performance Liquid Chromatography (HPLC) in the Analysis of Pharmaceutical Compounds
DOI:
https://doi.org/10.56359/igj.v5i2.959Keywords:
HPLC, Drug Analysis, HPLC Method ValidationAbstract
High Performance Liquid Chromatography (HPLC) is an essential analytical technique used for the separation, identification, and quantification of compound within complex mixtures.This review article examines several research studies discussing the application of HPLC in drug analysis. This review provide an overview of HPLC utilization across various studies, including its technical advantages, analytical challenges in diverse matrices, differences in column and mobile phase selection, variations in sample preparation techniques, detector performance, the influence of flow rate, analytical parameter quality, and method validation evaluation across different drug classes. This review is expected to provide a comprehensive understanding of HPLC application in pharmaceutical analysis and important considerations in developing accurate and reliable analytical methods.
Downloads
References
Agustina, R., Mulyadi, D., & Yuningsih, L. M. (2025). Optimization and Validation of Clobazam Content Determination Method in Tablet Preparations Using High Performance Liquid Chromatography (HPLC). Jurnal Biologi Tropis, 25(3), 4213-4220.
Alatas, F., Sujono, H., & Sucipto, W. A. (2018). Pengembangan dan validasi metode kromatografi cair kinerja tinggi (KCKT) untuk estimasi kadar simultan antiemetik piridoksin hidroklorida dan piratiazin teoklat dalam bentuk sediaan tablet. Kartika: Jurnal Ilmiah Farmasi, 6(2), 95-100.
Ali, A. H. (2022). High-performance liquid chromatography (HPLC): A review. Ann. Adv. Chem, 6, 010-020.
Arifah, R. H., Permatasari, D. A. I., & Artini, K. S. (2023). Penggunaan Metode HPLC pada Analisis Jamu Depot yang Mengandung Antalgin. Jurnal Jamu Kusuma, 3(1), 54-61.
Chrissanti, R. D., Darmawati, A., & Yuwono, M. (2020). Optimasi Metode KCKT untuk Penetapan Kadar 4-Isobutilasetofenon dan 2-(4-Isobutirilfenil) Asam Propanoat dalam Tablet Ibuprofen. Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia Vol, 7(1), 26.
Darmawati, A., Annuryanti, F., & Primaharinastiti, R. (2020). Determination and stability testing method of chlorpheniramine maleate in the presence of tartrazine using HPLC. Pharmaciana, 10(3), 269-280.
Dong, M. W., & Boyes, B. E. (2018). Modern trends and best practices in mobile-phase selection in reversed-phase chromatography. LC-GC Europe, 31, 572–583.
Fauziah, A. N., Kim, M. J., So, B. R., Son, J. E., & Jung, S. K. (2024). Validation of the High-Performance Liquid Chromatography Method for Determining Quercitrin in Capsicum annuum L. Cultivar Dangjo. Preventive nutrition and food science, 29(4), 504–511. https://doi.org/10.3746/pnf.2024.29.4.504
Febriani, R., Nurmalasari, A., Firdaus, N. R., & Setiawan, D. (2026). Stabilitas Spesimen Urine Positif Amfetamin yang Diawetkan dengan Kitosan. Borneo Journal of Medical Laboratory Technology, 8(2), 1231-1236.
Fernando, A., Putri, N. K., & Fadhli, H. (2024). Validasi Metode Penetapan Kadar Metformin Dalam Plasma Darah Yang Diberikan Bersamaan Dengan Pemberian Sari Buah Pare (Momordica charantia L.) Secara Kromatografi Cair Kinerja Tinggi (KCKT). Jurnal Penelitian Farmasi Indonesia, 13(1), 32-40.
Goutal, S. et al. (2016). Validation of a simple HPLC-UV method for rifampicin determination in plasma. Journal of Pharmaceutical and Biomedical Analysis, 123, 173–178.
Indriana, M. (2020). Determination of paracetamol levels in tablets and oral solutions by high-performance liquid chromatography (HPLC). Journal of Pharmaceutical And Sciences, 3(2), 106-113.
Kashmar, S. A., Abou Assi, R., Alhijjaj, M., & Chan, S. Y. (2025). Development and Validation of a Simultaneous HPLC Stability-Indicating Method for Atorvastatin and Apigenin in a Novel SMEDDS Formulation Using Quality by Design (QbD) Approach. Processes, 13(9), 2933.
Khatak, S. et al. (2018). Development and validation of RP-HPLC method for antitubercular drugs. Indian Journal of Pharmaceutical Sciences, 80, 996–1002.
Khodijah, S., Sriwidodo, & Roestan, R. (2024). Validasi metode analisis penetapan kadar Paracetamol, Chlorphenamine Maleate, Dextromethorphan HBr, dan Phenylephrine HCl dalam sediaan kaplet secara HPLC. Medic Nutricia: Jurnal Ilmu Kesehatan, 2(3), 91–109
Maulina, D., Asya, Z., Cahyo, D., Samira, S., & Mahmudah, W. (2024). Analisis Ibuprofen Menggunakan HPLC dan Pengujian Validasi Metode. Jurnal Ilmiah Wahana Pendidikan, 10(14), 390-423. https://doi.org/10.5281/zenodo.13580099
Mikulić, M., Sazdanić, D., Kladar, N., Radulović, J., Srđenović Čonić, B., & Atanacković Krstonošić, M. (2025). Validation of HPLC-DAD method for analysis of paracetamol and potassium sorbate in liquid oral formulations and its application to forced degradation study. Acta Chromatographica, 37(1), 112-120
Muharini, R., Ersando, E., Triana, Y., Nukila, M., Ulwan, R., Masriani, M., & Proksch, P. (2022). Analisis Profil HPLC-PDA Berkombinasi dengan LC-ESI-MS dan Aktivitas Biologi dari Ekstrak Jamur Endofit, Penicillium simplicissimum, yang Diisolasi dari Rimpang Jahe Merah. ALCHEMY Jurnal Penelitian Kimia, 18(2), 158-164.
Mulidini, M., Nanda, A. Y. D., Hanum, N. K., Nurfadhila, L., & Utami, M. R. (2023). Analysis and validation of metformin in human plasma using the HPLC method. Journal of Pharmaceutical and Sciences, 6(2), 741–749.
Nopi, N. S., & Isnaeni, N. (2023). Validasi Metode Analisis Penetapan Kadar Bahan Baku Lapatinib Ditosylate secara KCKT-DAD. Eruditio: Indonesia Journal of Food and Drug Safety, 4(1), 33-42.
Oliveira, M. A. L. et al. (2018). Simultaneous determination of antituberculosis drugs in FDC formulations: A review. Analytical Methods, 10, 1103–1116.
Patala, R., & Awilia, N. (2023). Analisis Kadar Parasetamol Generik Dibandingkan Bermerek Dagang. JIFI (Jurnal Ilmiah Farmasi Imelda), 7(1), 7-12.
Poole, C. (2009). Handbook of Methods and Instrumentation in Separation Science: Volume 1 (Vol. 1). Academic Press.
Pradana, D. A., Kristin, E., Nugroho, A. K., Nugrahaningsih, D. A. A., Mustofa, M., & Wibowo, A. (2023). Bioanalytical method Validation of metformin hydrochloride in human plasma by HPLC-UV for preliminary Population-Based Pharmacokinetic Modeling study. Indonesian Journal of Chemistry, 23(4), 937-947.
Prajapati, S. K., Jain, A., & Bajpai, M. (2024). Development and validation of the RP-HPLC method for quantification of tavaborole. Analytical Methods, 16(30), 5280-5287.
Priyadi, S., Darmadji, P., Santoso, U., & Hastuti, P. (2015). Distribusi plumbum, cadmium pada biji kedelai, dan deprotonasi gugus fungsional karboksil asam sitrat dalam khelasi. Agritech, 34(4), 407-414.
Putri, A. K., & Yuwono, M. (2023). Analisis Kualitatif dan Kuantitatif P-Aminophenol dalam Sediaan Sirup Parasetamol dengan Metode HPLC. Journal of Islamic Pharmacy, 8(1), 27-33.
Rohayati, A., Hasanah, A. N., Saptarini, N. M., & Aryanti, A. D. (2015). Optimasi kondisi pemisahan glibenklamid kombinasi metformin dalam plasma darah menggunakan KCKT. Indonesian Journal of Pharmaceutical Science and Technology, 2(3), 96.
Sangeetha, M., & Mahalakshmi, A. S. (2025). A Review on Analytical Method Development and Validation for the Simultaneous Estimation of Levothyroxine and Liothyronine in Combined Pharmaceutical Dosage Form by RP-HPLC. International Journal of Research in Pharmacy and Allied Science, 4(9), 54-60.
Sarılmışer, H. K., Özçelik, N. O., Arslan, B. Ö., Gökalp, M., Ustaoğlu, A., Dude, U. K., & Knezevic, Z. (2017). Development and validation of a simple HPLC assay method for ibuprofen, pseudoephedrine hydrochloride and chlorpheniramine maleate in syrup form. J. Chem. Metrol, 11(2), 61.
Setyaningrum, L. (2022). Validasi metode analisis penetapan kadar parasetamol dan kafein dalam sediaan tablet secara simultan menggunakan RP-HPLC. Jurnal Katalisator, 7(2), 323–335.
Suhendi, A., & Ramly, E. A. (2021). Validasi Metode Analisis Kapsul Rifampisin Dengan HPLC-PDA. Farmasains, 8(1), 31-36.Umar, S., Saafrida, S., & Lucida, H. (2021). Validasi Metoda Analisis Penetapan Kadar Ketoprofen pada Tablet Salut Enterik secara Kromatografi Cair Kinerja Tinggi dan Spektrofotometri UV. Jurnal Sains Farmasi & Klinis, 8(2), 200-207.
Umar, S., Saafrida, & Lucida, H. (2021). Validasi metoda analisis penetapan kadar ketoprofen pada tablet salut enterik secara kromatografi cair kinerja tinggi dan spektrofotometri UV. Jurnal Sains Farmasi & Klinis, 8(2), 200–207.
Woottisin, N., Kongkiatpaiboon, S., Sukprasert, S., & Sathirakul, K. (2020). Development and validation of stability indicating HPLC method for determination of caffeic acid, vitexin and rosmarinic acid in Thunbergia laurifolia leaf extract. Pharmacognosy Journal, 12(3).
Yashin, Y. I., & Yashin, A. Y. (2020). Contribution of Russian Experts to the Development of High-Performance Liquid Chromatography. Journal of Analytical Chemistry, 75(10), 1252-1263.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Verna Wimala Salsabila, Elina Jatindria, Rimadhyna Widya Khairunissa, Athaya Wijdan Pascadiksa, Elvina Putri Rahmawati, Nur’Aini Hidayati, Aldo Octavian Ramadhan Saputra, Djustike Maylasari
This work is licensed under a Creative Commons Attribution 4.0 International License.
