Quantitative Comparison of Soil Transmitted Helminths (STH) Eggs Flotation Method with Different Solutions and Times

Authors

  • Ardian Chairil Anam Akademi Analis Kesehatan Pekalongan
  • Abdul Ghofur Akademi Analis Kesehatan Pekalongan

DOI:

https://doi.org/10.56359/igj.v4i2.605

Keywords:

Soil Transmitted Helminths, Flotation, Solution, Incubation

Abstract

Background & Objective: Soil-Transmitted Helminths (STH) infections are among the most common communicable diseases worldwide and have a significant impact on public health. These infections are caused by several types of intestinal worms, including Ascaris lumbricoides, Trichuris trichiura, Strongyloides stercoralis, Necator americanus, and Ancylostoma duodenale. Children and individuals working in agriculture are particularly at high risk of infection. The flotation method is commonly used to detect helminth eggs in stool samples. This study aimed to assess the effectiveness of the flotation method using different solutions—NaCl, ZnSO₄, and MgSO₄—at incubation times of 30 minutes and 40 minutes in detecting the number of STH eggs. Method: This study used an experimental design, where the researchers applied different flotation solutions and incubation times. The results were statistically analyzed using the MANOVA test. Results: The MANOVA test showed that all significance values were > 0.05, indicating no statistically significant effect of the different flotation solutions and incubation times on the quantitative detection of STH eggs. However, descriptive analysis revealed that the ZnSO₄ solution with 40 minutes of incubation was more effective than NaCl and MgSO₄, as it detected a higher number of STH eggs. Conclusion: It can be concluded that there is no statistically significant effect of the flotation method using different solutions and incubation times on the quantitative detection of Soil-Transmitted Helminth eggs. Nonetheless, ZnSO₄ with 40 minutes of incubation showed relatively better descriptive results.

Downloads

Download data is not yet available.

References

Ariyadi, R., Ruhimat, U., Gantina, H. T. S., & Firdaus, N. R. (2024). Identifikasi Nematoda Usus Pada Balita Stunting di Wilayah Kerja Puskesmas Baregbeg. INDOGENIUS, 3(3), 177-181.

Islamiati, D., Basarang, M., Rianto, M. R., & Widyanti, T. (2021). Identifikasi Nematoda Usus Soil Transmitted Helminth Pada Anak-Anak Di Kecamatan Baranti Kabupaten Sidenreng Rappang. Lontara Abdimas : Jurnal Pengabdian Kepada Masyarakat, 2(1), 21–25. https://doi.org/10.53861/lomas.v2i1.201

Kemenkes. (2022). Pengaruh cacingan pada kesehatan anak. RSUP Dr. Soeradji Tirtonegoro Klaten, 1–6. https://yankes.kemkes.go.id/view_artikel/1554/pengaruh-cacingan-pada-kesehatan-anak

Nikmatullah, N. A., Wijiastuti, & Rahmadilla, C. (2023). Efektifitas Larutan ZnSO4 33% dan Sukrosa 33% Pada Pemeriksaan Soil Transmitted Helminth Menggunakan Metode Flotasi Sentrifugasi. The Journal of Muhammadiyah Medical Laboratory Technologist, 6(2), 152–159. https://doi.org/10.30651/jmlt.v6i2.18289

Novianty, S., Pasaribu, H. S., & Pasaribu, A. P. (2019). Faktor Risiko Kejadian Kecacingan pada Anak Usia Pra Sekolah. Journal Of The Indonesian Medical Association, 68(2), 86–92. https://doi.org/10.47830/jinma-vol.68.2-2018-91

Nurhidayanti, & Indah Sari. (2023). Pemeriksaan Mikroskopis Kualitas Sediaan Telur Cacing Trichuris trichiura Menggunakan Metode Natif Dan Metode Flotasi. Journal Health Applied Science and Technology, 1(2), 28–33. https://doi.org/10.52523/jhast.v1i2.16

Rahayu, A. T., Pratama, A., Setiawan, M. W., Ma’rifatussolihat, & Nikmatullah, N. A. (2021). Optimasi Metode Flotasi Sentrifus Menggunakan Larutan ZnSO4, MgSO4, DAN NaCl Berdasarkan Konsentrasi Larutan Dan Lama Pengapungan. Jurnal Media Analis Kesehatan, 14(1). https://doi.org/10.32382/mak.v14i1.3283

Ruhimat, U., Mutmainah, R. N., Ramdan, S. R. K., & Firdaus, N. R. (2024). Utilization of Purple Sweet Potato Juice As an Alternative Violet Crystal in Coloring Streptococcus sp Bacteria and Escherichia coli. JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS, 11(2), 149-156.

Regina, M. P., Halleyantoro, R., & Bakri, S. (2018). Perbandingan Pemeriksaan Tinja Antara Metode Sedimentasi Biasa Dan Metode Sedimentasi Formol-Ether Dalam Mendeteksi Soil-Transmitted Helminth. Diponegoro Medical Journal (Jurnal Kedokteran Diponegoro), 7(2), 527–537.

Rusyda, M. N. (2023). Pengaruh Perbedaan Variasi Waktu Flotasi Menggunakan Larutan MgSO4 dan NaCl Terhadap Hasil Pemeriksaan Telur Cacing Soil Transmitted Helminths. http://repo.upertis.ac.id/id/eprint/3286

Steinbaum, L., Kwong, L. H., Ercumen, A., Negash, M. S., Lovely, A. J., Njenga, S. M., Boehm, A. B., Pickering, A. J., & Nelson, K. L. (2017). Detecting and enumerating soil-transmitted helminth eggs in soil: New method development and results from field testing in Kenya and Bangladesh. PLoS Neglected Tropical Diseases, 11(4), 1–15. https://doi.org/10.1371/journal.pntd.0005522

Wasilah, N., Sulaeman, Mulia, Y. S., & Solihat, M. F. (2024). Perbandingan Pemeriksaan Tinja Antara Metode Flotasi Dan Metode Ritchie Dalam Mendeteksi Soil Transmitted Helminth. Jurnal Kesehatan Siliwangi, 5(2), 374–382. https://doi.org/10.34011/jks.v5i2.2382

WHO. (2023). Infeksi Cacing yang Ditularkan Melalui Tanah. Who.Int, 1–5. https://www.who.int/news-room/fact-sheets/detail/soil-transmitted-helminth-infections

Downloads

Published

2025-05-28

How to Cite

Chairil Anam, A., & Ghofur, A. (2025). Quantitative Comparison of Soil Transmitted Helminths (STH) Eggs Flotation Method with Different Solutions and Times. INDOGENIUS, 4(2), 250–255. https://doi.org/10.56359/igj.v4i2.605

Similar Articles

You may also start an advanced similarity search for this article.